Pacific blue fluorophore
Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ... Comparable Fluorophores: Pacific Blue™, Alexa Fluor® 405, eFluor® 450, BD Horizon™ V450, Cascade Blue™ Brightness = 5 (On a scale from 1 to 5, with 5 being the brightest.) Molar Extinction Coeff. = 2,500,000 M-1cm-1 Quantum Yield = 0.65 in DPBS Beta Testing - Resolution of mouse NK1.1 populations; High sensitivity for mouse CD127 We report a new method to quantify the affinity of small molecules for proteins. This method is based on Förster resonance energy transfer (FRET) between endogenous tryptophan (Trp) residues and the coumarin-derived fluorophore Pacific Blue (PB). Tryptophan residues are frequently found in proteins near ligand-binding sites, making this approach potentially applicable to a wide range of ...
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The LIVE/DEAD™ Fixable Blue Stain was selected based on its fluorescent properties to provide a bright signal when excited with a UV laser. The blue-fluorescent reactive dye has an excitation maximum of ∼350 nm, making it ideal for use with an UV laser, and an emission of ∼450 nm. By switching viability staining from a channel off of the ...Common fluorophores used with laser; Ultraviolet (UV) 355 nm: DAPI, Hoechst, LIVE/DEAD Blue, Brilliant Ultraviolet: Violet: 405–407 nm: Pacific Blue, eFluor 450, Pacific Orange, eFluor 506, Super Bright 436, Super Bright 600, Brilliant Violet, LIVE/DEAD Yellow, LIVE/DEAD Aqua, LIVE/DEAD Violet, CFP : Blue: 488 nmBlue BD Horizon V450 Pacific Blue™ BD Influx™ cell sorter Solid State 488 Green FITC Alexa Fluor® 488 Yellow PE Orange PE-Texas Red® Red PE-Cy5 PerCP-Cy5.5 Infrared PE-Cy7 Solid State 532 or 561 Yellow PE Orange PE-Texas Red® Red PE-Cy5 Infrared PE-Cy7 Solid State 640 Red APC Alexa Fluor® 647 Far Red Alexa Fluor®700 Infrared BD APC-H7 ...
Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ...Reagent Selection Guide for the Attune Flow Cytometers. The Invitrogen Attune cytometers can be designed to accommodate the most common fluorophores and fluorescent proteins used in flow cytometry, to match the panels you are currently running. Multiple fluorescent proteins can be interrogated with the 4-laser version of the Attune Flow Cytometers.Quantification of Small Molecule-Protein Interactions using FRET between Tryptophan and the Pacific Blue Fluorophore. ...The traditional blue-green 488 nm laser line produced by argon-ion sources was and remains (via newer solid state sources) the primary laser line for most flow cytometric analysis. The 488 nm line excites fluorescein, a low molecular weight fluorochrome that forms the molecular basis for a wide variety of fluorescent cell tags and physiological ...Alexa Fluor 488 Dye. Invitrogen Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 488 dye is pH-insensitive over a wide molar range. We offer Alexa Fluor 488 dye conjugated to a variety of antibodies, peptides ...
Fluorescence Emission Fluorochrome Color Ex-Max (nm) Analyzers Sorters Excitation Laser Line (nm)† Em-Max (nm) BD Life Sciences Fluorochrome Specifications * Capable of detecting up to 8 colors simultaneously (488-nm laser available on all configurations; 405-nm and 640-nm lasers available on select configurations). ** Capable of detecting up to 12 colors …Blue (488 nm) BD Horizon™ BB515 BD Horizon™ PE-CF594 PE-Cy™5 PE PE-Cy™7 FITC Alexa Fluor® 488 PerCP-Cy™5.5 PerCP Yellow/Green (561 nm) PE BD Horizon PE-CF594 ... Many factors can influence the relative fluorochrome/reagent performance on a given instrument, including laser power, PMT voltage, optical filters, ant ibody clone, and ...Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ... ….
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Pacific Orange dye is a bright orange-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 400/551 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm ...The Alexa Fluor®-labeled antibodies can be used for multiple applications, including fluorescent microscopy, flow cytometry, Western blotting, ELISA, and indirect FISH. Pacific BlueTM and Pacific OrangeTM are unique dyes designed for use with a violet laser (405 nm) for flow cytometry applications.
Fluorophore at a Glance: An upgrade over FITC in terms of brightness. A bright and spectrally clean fluorophore that only exhibits significant spillover into Spark Blue™ 550. Few, if any, fluors spill into KIRAVIA Blue 520™. Utilize this fluor on ubiquitously expressed markers or markers with varying levels of expression. Fluorophore type. Organic dye. Organic dye. Organic dye (tandem), resonance energy transfer from PE molecule to eFluor 610 dye. Organic dye (tandem), resonance energy transfer from PerCP molecule to eFluor 710 dye. Ex (nm) 475. 546. 496, 565. 488. Em (nm) 535. 585. 607. 710. Application. ICC, IHC. ICC, IHC. FC. FC. Conjugates available : eFluor ...
siena mbb The Pacific Northwest is a region of unparalleled natural beauty, with its rugged coastlines, towering mountains, and lush forests. For those seeking a unique and immersive way to experience this stunning landscape, Pacific Northwest river ...Fluorophore at a Glance: An upgrade over FITC in terms of brightness. A bright and spectrally clean fluorophore that only exhibits significant spillover into Spark Blue™ 550. Few, if any, fluors spill into KIRAVIA Blue 520™. Utilize this fluor on ubiquitously expressed markers or markers with varying levels of expression. rice university volleyball scheduleku vs mizzou football Alexa Fluor dyes—Emission spectra. Figure 1. Emission spectra for Alexa Fluor dyes. Table 1. Properties of Alexa Fluor Dyes. * Typical emission color seen through the eyepiece of a conventional fluorescence microscope with appropriate filters. ** Extinction coefficient at lmax in cm–1M–1. *** Human vision is insensitive to light beyond ... wichita illinois 441. (in buffer): 1. (in antifade): 2. microscopy, flow cytometry, fluorescence microplate reader. Invitrogen Alexa Fluor 350 dye is a blue-fluorescent dye with moderate photostability, and excitation that matches the 350 nm laser line. The brightness and photostability of blue dyes are best suited to direct imaging of high-abundance targets. Some reagents must be diluted for optimal staining. It is recommended that the investigator determine the dilution that is optimal for each assay. Pacific Blue™ ... witchiapre bis resto druid wotlkkansas basketball 2021 StarBright Blue 810. 477. 802. 4. 750LP. 750LP. A488 (ex max 495, ex max 419) With similar fluorescence properties to FITC, Alexa Fluor 488 is not compatible with FITC or GFP. However, as it is brighter and more photostable, it is a great alternative, especially for intracellular staining and in fluorescence microscopy. devilliers Structure-guided engineering of a Pacific Blue fluorophore ligase for specific protein imaging in living cells 2011 Sep 27;50 (38):8221-5. doi: 10.1021/bi201037r. Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA. PMC3181222 10.1021/bi201037r Catalytic Domain / genetics cooper kellywalter knolls flowersgreg marshal Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ...